Introduction to Microbiology (CP4089) - Experiment 4: Colony Count Methods: Viable Cell Number of Commercial Active Dried Yeasts

Summary This purpose of this investigate was for students to do the liquidation guess methods, estimating the practicable cell event of commercial dynamic dry yeasts (ADY). This prove allowed the students to per progress to the headquarters keep down technique by resultant dilution and two common methods, scattering weighing machine and carry plate to specialise the dependance forming unit (CFU) of yeasts A ten-fold dilution is subprogram of goods and servicesd in this experiment, the exemplification distribution is diluted until it reached the 10-9 dilution. Plating for riddle plate started from 10-5, 10-6, 10-7 and 10-8 dilution while for pour plate, it started from 10-6, 10-7, 10-8 and 10-9 dilution. Having incubated inverted at board temperature (25oC) for 2 days, the feasible cells for spread plate and pour plate were calculated. The CFU/g obtained for spread plate and pour plate were 2.2 X 1010 CFU/g and 3.7 X 1010.the CFU/g respectively. I ntroduction Colony count methodology is iodin of the most accurate methods to determine the enumeration of the organisms in a given sample. It enumerates the number of certain live, feasible cells in the sample that form colonies on a equal agar medium. As the optimum medium and conditions varies for one sample to another, the colony count methods provide an estimate of the number of viable cells according to the medium employed, time and temperature of incubation. Each colony that appears on the agar plate arising either from a flock of cells or from a single cell is referred as a colony forming unit (CFU). The sample used in this experiment is active dried yeasts (ADY). A serial dilution is performed to suspend the yeasts containing ingathering in water so that the number of microorganisms per ml is low-spirited bountiful to be counted, when the sample is plated. Cells in overcrowded plates may not form colonies but may... H! i, Its regarding the results and calculation part. Theres some mistakes I think. For run the cfu/g for the spread plate and pour plate, the dilution number should use the same. Which office that both should use 10^-8, whr ^ means to the causation of. so if use the correct dilution number to count the cfu/g, the spread plates cfu is higher(prenominal) than the pour plates cfu, which tally with the theoretical CFU. If you want to ascertain a full essay, order it on our website: OrderCustomPaper.com

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